Clinical & Pharmaceutical Solutions through Analysis
Where Technology and Solutions Meet

CPSA 2011

Science and Technology Coming Together to Make a Difference

October 3 - 6, 2011
Sheraton Bucks County Hotel
Langhorne, PA

Poster Session

Tuesday, October 4
1:15 PM - 3:00 PM



Poster #01
ADDA: A new LC/MS/MS sample delivery platform for drug discovery bioanalysis

John Janiszewski1, Rick Schneider1, Veronica Zelesky1, Brendon Kapinos1, Hui Zhang1, Matt Troutman1, Nick Levitt2, Jack Zhou2, Wayne Lootsma3, Will Schramm3 and Felix Yiu2
1) Pfizer Global Research and Development, Groton, CT USA; 2) Apricot Designs, Covina, CA USA; 3) Sound Analytics, Niantic, CT USA

Poster #02
Qualification of a Robotic Liquid Handler to Perform Bioanalytical Validation or Sample Analysis Runs

Ron Shoup
AIT Bioscience, LLC, Indianapolis, IN USA

Poster #03
Achieving Regulatory Approval: Overcoming Bioanalytical Challenges in an Onglyza™ Micro-tracer Absolute Bioavailability Study with Accelerator Mass Spectrometry

Xiaohui (Sophia) Xu1, Lisa J. Christopher1, Stephen Dueker2, Pete Lohstroh2, Vikram Roongta3, Laura Cojocaru4, Chi Fung (Anther) Keung1, W. Griffith Humphreys1, Bruce Stouffer1 and Mark Arnold1
1) Bristol-Myers Squibb, Princeton, New Jersey USA; 2) Vitalea Sciences, Inc, Davis, CA USA; 3); 4) Tandem Labs, West Trenton, NJ USA

Poster #04
Comparison of Microsampling Techniques for Quantitative DBS and DPS Preparation

Jim Kenney1, Joe Siple1, Chris Strohsahl2
1) Drummond Scientific Company; 2) University of Rochester

Poster #05
Development of a bioanalytical DBS assay using a tandem quadrupole with a novel collision cell design

Joanne Mather, Paul Rainville, Debadeep Bhattacharya, and Rob Plumb
Waters Corporation, Milford, MA

Poster #06
Comprehensive Study Examining Interfering Compounds from Various Dried Blood Spot Cards and Extraction Solvents with ESI Positive Tandem Quadrupole MS

Jennifer Simeone1; Chester L Bowen2; Joanne Mather1; Robert Plumb3; Debadeep Bhattacharya1; Paul Rainville1
1) Waters Corporation, Milford, MA USA; 2) GlaxoSmithKline, King Of Prussia, PA USA; 3) Imperial College, London, United Kingdom


Poster #07
LC/MS/MS Quantification of Fluticasone Propionate in Human Plasma at the fg/mL Level

Joanne Mather, Kendon Graham, Debadeep Bhattacharya, and Rob Plumb
Waters Corporation, Milford, MA

Poster #08
A Direct LC-MS/MS Method for Determination of ß-Alanine in Human Plasma

Qian (Cynthia) Chen, Connell Cunningham, Julie E. Eble
Critical Path Services, Garnet Valley, PA USA

Poster #09
The Separation of Basic Analytes on a Polar-modified C18 HPLC Stationary Phase

David W. House, Asha A. Oroskar, and Anil R. Oroskar
Orochem Technologies, Inc.

Poster #10
"Mass Spec friendly" Plastics Safe for Quantitative Mass Spectrometry Analysis

Igor Gavin, Naveen Sudharsan, Deepak Sharma, Asha A. Oroskar, and Anil R. Oroskar
Orochem Technologies Inc., Lombard, IL USA

Poster #11
A Novel High Throughput Method Using Full Scan HRAM and Online Extraction for Plasma Protein Binding Determination

Kevin Cook, Mark Dreyer, Keeley Murphy, Nicholas Duczak
Thermo Fisher Scientific, San Jose, California, USA

Poster #12
Quantitation of metabolites in plasma samples by UV-MS correction using a dual-cell-linear ion trap mass spectrometer

Tim Stratton, Yingying Huang, August Specht, Julie Horner
Thermo Fisher Scientific, San Jose CA USA

Poster #13
Optimization of Nanospray Voltage and Spray Stability: Impact on Chromatographic Peak Area for Analyte Quantitation

Amanda Berg1; Mike S. Lee2; Gary Valaskovic1
1) New Objective, Inc., Woburn, MA USA; 2) Milestone Development Services, Newtown, PA USA

Peptides & Proteins

Poster #14
Evaluation of 150 µm ID Packed Tip Columns for Quantitative Peptide Analysis by LC-MS/MS

Stanley Durand; Helena Svobodova; Amanda Berg; Gary Valaskovic
New Objective, Inc., Woburn, MA USA

Poster #15
Development and Validation of a Method for the Determination of a Genetic Fusion Protein in Human Plasma using UHPLC-MS/MS for First Time in Human (FTIH) Study Support

Bonnie Orr, Matthew Szapacs, Jonathan Kehler, Chester Bowen
GlaxoSmithKline Pharmaceuticals, King of Prussia, PA USA

Poster #16
Multiplexing SIMs on a Novel Benchtop Orbitrap MS with a Quadrupole Mass Filter to Increase Sensitivity for Peptide Quantitation

Kevin L. Cook, Patrick Bennett, Nicholas Duczak, Louis Maljers
Thermo Fisher Scientific, San Jose, California, USA

Biomarkers & Metabolomics

Poster #17
Analysis of a Therapeutic Antibody and a Biomarker in Parallel Utilizing Immunocapture and LC-MS/MS

Neha Akella, Jonathan Kehler, Matthew Szapacs and Scott Hottenstein
GlaxoSmithKline Pharmaceuticals, King of Prussia, PA USA

Poster #18
A Sensitive Assay for the Measurement of Endogenous Levels of Angiotensin Peptides as a Biomarker Utilizing HPLC-MS/MS Detection

Jonathan Kehler, Matthew Szapacs and Neha Akella
GlaxoSmithKline Pharmaceuticals, King of Prussia, PA USA

Poster #19
Overcoming the challenges in targeted and non-targeted metabolite profiling of myxobacterial secondary metabolites using UHR-Q-TOF-MS analysis

Daniel Krug1; Nina Cortina1; Gabriela Zurek2; Aiko Barsch2; Verena Tellström2; Rolf Mueller1
1) University of Saarbruecken, Saarbruecken, Germany; 2) Bruker Daltonik, Bremen, Germany

Characterization & Identification

Metabolite Identification

Poster #22
Using UPLC-QuanTOF technologies and chemically intelligent software tools for confident metabolite identification and biotransformation localization in discovery and development

Mark D. Wrona1, Stephen McDonald1, Jeff Goshawk2, Alan Millar1
1) Waters Corporation, Milford, MA USA; 2) Waters Corporation, Manchester, United Kingdom

Poster #23
Identification of a complicated metabolite for a GSK drug candidate using the structure identification tool box

Min Lin, Jill Pirhalla, Steve Castellino
GlaxoSmithKline, King of Prussia, PA USA

Poster #24
High-resolution accurate mass-measurements and metabolite identification: an automated approach using fragment prediction in combination with fragment ion search (FISh)

Paul-Gerhard Lassahn1, Rose Herbold2, Tim Stratton2
1) Swiss BioAnalytics AG, Birsfelden, Switzerland, 2) Thermo Fisher Scientific, San Jose CA USA

Poster #25
Application of a Novel Bench-top Orbitrap Mass Spectrometer with a Quadrupole Mass Filter for Metabolite Profiling in Drug Discovery

Qian Ruan1; Kate Comstock2; Li Ma1; Tim J Stratton2; Yingying Huang 2; Mingshe Zhu1
1) Bristol-Myers Squibb, Princeton, NJ USA; 2) Thermo Fisher Scientific, San Jose CA USA


Poster #26
By-products in recombinant TAU protein production characterized by LC-MALDI Top-Down Sequencing (LC-MALDI-TDS)

Arndt Asperger1, Marcus Macht1, Branislav Kovacech2, Andrej Kovac2
1) Bruker Daltonik, Bremen, Germany; 2) Institute of Neuroimmunology of Slovak Academy of Sciences, Bratislava, Slovakia

Poster #27
Development of a Methodology for Rapid Characterization of Biological Therapeutics

M. Willetts1, G. Tremintin2, C. Albers3, L.C. Main4, C. Baessmann3, W. Jabs3
1) Bruker Daltonics, Billerica, MA; 2) Bruker Daltonics, Fremont, CA; 3) Bruker Daltonik, Bremen, Germany; 4) Bruker Daltonics, Coventry, UK

Poster #28
Automatic MS/MS characterization of N-linked Glycopeptides

Andrea Kiehne, Anja Resemann, Ulrike Schweiger-Hufnagel, Arndt Asperger, Detlev Suckau
Bruker Daltonik, Bremen, Germany

Qual / Quan

Poster #20
Comparison of Accurate Mass and Nominal Mass MSMS for the Simultaneous Acquisition of Qualitative and Quantitative Data in DMPK Studies

Joanne Mather, Marian Twohig, Hilary Major, Paul Rainville, Debadeep Bhattacharya, and Robert S. Plumb
Waters Corporation, Milford, MA, USA

Poster #21
Simultaneous Determination of Metabolic Stability, Metabolite Identification and Profiling Using the Agilent 6550 iFunnel Q-TOF LC/MS System

Yuqin Dai, Michael Flanagan, and Keith Waddell
Agilent Technologies, Inc., Santa Clara, CA USA


Poster #29
A simple and effective tube construct for salty samples in cryoprobes

István Pelczer
Princeton University, Princeton, NJ USA

Poster #30
Fundamental Characteristics of Small Molecule Analysis using Ultra Low Dynamic Flow

Ben Ngo1; Shaoxia Yu2; Mike S. Lee3; Jing-Tao Wu2; Gary Valaskovic1
1) New Objective, Inc., Woburn, MA USA; 2) Millennium Pharmaceuticals, Inc., Cambridge, MA USA; 3) Milestone Development Services, Newtown, PA USA

Poster #31
High Throughput Screening for Lead Discovery using Multiplexed LC with High Resolution Accurate Mass LC/MS

Keeley Murphy, Nick Duczak, Francois Espourteille
Thermo Fisher Scientific, San Jose, CA USA

Poster #32
Rapid Chemical ID using a Direct Analysis in Real Time Mass Spectrometry using a Heated Sample Screen in the ID-CUBE™

Brian Musselman1, Jordan Krechmer1, Joseph Tice1, and Zhongli Zhang2
1) IonSense, Inc., Saugus, MA USA; 2) IonSense, Inc. Visiting Scientist

Data Analysis

Poster #33
Using Electronic Lab Notebook Functionality for Validating Electronic Lab Notebook Workflows

Brian Beato, Tim Grever and Laurel Branstrator
AIT Bioscience, Indianapolis, IN USA

Poster #34
Strategy for Designing Electronic Lab Notebook Workflows Both Flexible Enough for R&D and Comprehensive Enough for Regulated Work

Brian Beato, April Pisek and Jessica White
AIT Bioscience, Indianapolis, IN USA

Poster #35
Efficient Strategy for Generating Method-Specific Workflows in an Electronic Lab Notebook

Brian Beato, Jessica White and April Pisek
AIT Bioscience, Indianapolis, IN USA

Poster #36
Advances in the Automated Data Analysis of High Resolution Accurate Mass LC-MS Metabolomics

Serhiy Hnatyshyn1, Petia Shipkova2, Michael Reily1, Mark Sanders3
1) Bristol-Myers Squibb Co., Lawrenceville, NJ, USA; 2) Bristol-Myers Squibb Co., Pennington, NJ, USA; 3) Thermo Fisher Scientific, Somerset, NJ USA

Poster #37
Automated Deisotoping and Charge Deconvolution of High-Resolution LCMS Data

Mark E. Hail1 and Stuart Ray2
1) Novatia, LLC, Monmouth Junction, NJ; 2) Positive Probability, Ltd., UK

Poster #38
Mass Spectral Structure Elucidation and Correlation Algorithm Based Upon Advanced Graph Theory Methods

Marshall M. Siegel
MS Mass Spectral Consultants, Fair Lawn, NJ USA

CPSA - Where Technology and Solutions Meet

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